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Fractions were buffer exchanged into PBS, concentrated, passed through a 0.2 m filter, and quantified by measuring the absorbance at 280 nm using a Nanodrop One (ThermoFisher Cat

Simplistic conventional, also inappropriately called 2D, monolayer cultures with a single layer of cells attached to treated polystyrene have unnaturally low cell densities, lack cell-cell interaction or cell-peri and extracellular matrix interactions, and lack the exhibition of the brain tumor nutrient gradients or physiological levels of oxygen (112)